IF;WB;IHC;ELISA

The antiserum was produced against synthesized peptide derived from human RhoA. AA range:144-193

Rho A Polyclonal Antibody detects endogenous levels of Rho A protein.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; Not yet tested in other applications; Note:For IHC,wesuggest antigen retrieval with TE buffer pH 9.0

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

1 mg/ml

cofactor:Magnesium.,Domain:The basic-rich region is essential for yopT recognition and cleavage.,Function:Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.,PTM:Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.,PTM:Substrate for botulinum ADP-ribosyltransferase.,similarity:Belongs to the small GTPase superfamily. Rho family.,subunit:Interacts with RGNEF (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation.,

RHOA

Transforming protein RhoA

ARHA;ARH12;RHO12;EDFAOB;RHOH12;RhoA;Transforming protein RhoA;Rho cDNA clone 12;h12

387

P61586

11848

Q9QUI0

117273

P61589

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Cell projection, dendrite . Nucleus . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner. .

Adipose tissue,Brain,Colon,Mammary cancer,Oesophageal carcinoma,Placenta

-15°C to -25°C/1 year(Do not lower than -25°C)