免疫原
The antiserum was produced against synthesized peptide derived from human ACTN1/2/3/4. AA range:21-70
特异性
Actinin-α1/2/3/4 Polyclonal Antibody detects endogenous levels of Actinin-α1/2/3/4 protein.
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
功能
function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,
基因名称（Gene Name）
ACTN1/2/3/4
蛋白名称
Alpha-actinin-1/2/3/4
其他名称
actinin 1 smooth muscle;Actinin alpha 1;actinin;alpha 1;ACTN 1;Actn1;ACTN1_HUMAN;Alpha Actinin 1;Alpha actinin cytoskeletal isoform;Alpha-actinin cytoskeletal isoform;Alpha-actinin-1;BDPLT15;F actin cross linking protein;F-actin cross-linking protein;FLJ4884;FLJ54432;Non muscle alpha actinin 1;Non-muscle alpha-actinin-1;Actin binding protein;Actinin alpha 2;ACTN 2;ACTN2;ACTN2_HUMAN;Alpha actinin 2;Alpha actinin skeletal muscle;Alpha actinin skeletal muscle isoform 2;Alpha-actinin skeletal muscle isoform 2;Alpha-actinin-2;CMD1AA;Actinin alpha 3;alpha;ACTN 3;ACTN3;ACTN3_HUMAN;Alpha actinin skeletal muscle isoform 3;Alpha-Actinin 3;Alpha-actinin skeletal muscle isoform 3;Alpha-actinin-3;MGC1172;MGC1175;OTTHUMP21949;OTTHUMP21954;actinin 4;Actinin alpha 4;actinin4;ACTN 4;ACTN4;ACTN4_HUMAN;Alpha-actinin-4;DKFZp686K23158;Focal segmental glomerulosclerosis 1;FSGS 1;FSGS;FSGS1;Non muscle alpha actinin 4;Non-muscle alpha-actinin 4;Actinin-α1/2/3/4;ACTN1/2/3/4;Alpha-actinin-1/2/3/4;A
Fields
>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Shigellosis;>>Amoebiasis;>>Viral carcinogenesis;>>Systemic lupus erythematosus
人蛋白质序列数据库
P12814/P35609/Q08043/O43707
小鼠基因ID
109711/11472/11474/60595
细胞定位
Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cell membrane . Cell junction . Cell projection, ruffle . Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. .
组织表达
Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue,
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