免疫原
Synthesized peptide derived from the Internal region of human PKN.
特异性
PKN Polyclonal Antibody detects endogenous levels of PKN protein.
组成（Formulation）
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
WB 1:500-2000;IHC-p 1:50-300
纯化工艺（Immunogen）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
背景（Background）
protein kinase N1(PKN1) Homo sapiens The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.,function:Can phosphorylate ribosomal protein S6. Mediates GTPase Rho dependent intracellular signaling.,PTM:Activated by limited proteolysis with trypsin.,PTM:Autophosphorylated; preferably on serine.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 REM (
蛋白名称
Serine/threonine-protein kinase N1
其他名称
DBK;PKN;PAK1;PRK1;PAK-1;PRKCL1;PKN-ALPHA;PKN1;Serine/threonine-protein kinase N1;Protease-activated kinase 1;Protein kinase C-like 1;Protein kinase C-like PKN;Protein kinase PKN-alpha;Protein-kinase C-related kinase 1;Serine-threonine protein
Fields
>>PI3K-Akt signaling pathway;>>NOD-like receptor signaling pathway;>>Salmonella infection;>>Yersinia infection
细胞定位
Cytoplasm . Nucleus . Endosome . Cell membrane ; Peripheral membrane protein . Cleavage furrow . Midbody . Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. .
组织表达
Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
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