背景(Background)
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
免疫原
Synthetic Peptide of Flag-Tag
特异性
The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.
组成(Formulation)
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
稀释比例
WB: 1:1000-5000 ELISA 1:20000-50000 IF: 1:100-300
纯化工艺(Immunogen)
Protein A purified