背景（Background）
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
免疫原
Synthetic peptide:HHHHHH conjugated to KLH.
特异性
The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
组成（Formulation）
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
稀释比例
WB: 1:3000 IP: 1:200 IF: 1:1000
纯化工艺（Immunogen）
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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