背景(Background)
The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.
免疫原
HA synthetic peptide conjugated to KLH.
特异性
HA-tag Polyclonal Antibody detects HA-tagged recombinant proteins or HA-tagged proteins overexpressed in cells.
组成(Formulation)
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
稀释比例
Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications.
纯化工艺(Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.