免疫原
Purified recombinant human ATP-citrate synthase (C-terminus) protein fragments expressed in E.coli.
特异性
ATP-citrate synthase Monoclonal Antibody detects endogenous levels of ATP-citrate synthase protein.
组成（Formulation）
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
稀释比例
Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Flow cytometry: 1/100 - 1/200. Not yet tested in other applications.
纯化工艺（Immunogen）
Affinity purification
背景（Background）
ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],
功能
catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer.,
蛋白名称
ATP-citrate synthase
其他名称
ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme
Fields
>>Citrate cycle (TCA cycle);>>Metabolic pathways
细胞定位
Cytoplasm, cytosol .
组织表达
Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet,
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