免疫原
Purified recombinant human Smad5 (C-terminus) protein fragments expressed in E.coli.
特异性
Smad5 Monoclonal Antibody detects endogenous levels of Smad5 protein.
组成（Formulation）
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
稀释比例
Western Blot: 1/1000 - 1/2000. Immunofluorescence: 1/100 - 1/500. Flow cytometry: 1/100 - 1/200. Not yet tested in other applications.
纯化工艺（Immunogen）
Affinity purification
背景（Background）
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
功能
function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.,tissue specificity:Ubiquitous.,
蛋白名称
Mothers against decapentaplegic homolog 5
其他名称
SMAD5; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Fields
>>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
联系我们
关注我们
您当前的位置： 
说明书
成功添加到购物车
